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Image Search Results
Journal: PLOS One
Article Title: Simultaneous efficacy of IL-15 and the HSP70 fragments in increasing immunostimulatory effects of a HIV-1 DNA vaccine candidate against SCR HIV-1
doi: 10.1371/journal.pone.0336042
Figure Lengend Snippet: Cytokine profiling of IFN-γ and IL-5 secreted by splenocytes re-stimulated with recombinant proteins: A–B) Re-stimulation with Nef; C–D) Re-stimulation with NT-Hsp70-Nef, CT-Hsp70-Nef, or IL-15. Cytokine levels were measured by sandwich ELISA in duplicate assays per group (ns: non-significant; * p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Article Snippet: The concentrations of interferon-gamma (IFN-γ) and interleukin-5 (IL-5) in culture supernatants were quantified using commercial
Techniques: Recombinant, Sandwich ELISA
Journal: PLOS One
Article Title: Simultaneous efficacy of IL-15 and the HSP70 fragments in increasing immunostimulatory effects of a HIV-1 DNA vaccine candidate against SCR HIV-1
doi: 10.1371/journal.pone.0336042
Figure Lengend Snippet: Data represent sandwich ELISA results performed in triplicate per sample (ns: non-significant; * p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Article Snippet: The concentrations of interferon-gamma (IFN-γ) and interleukin-5 (IL-5) in culture supernatants were quantified using commercial
Techniques: Sandwich ELISA
Journal: Research
Article Title: Spatiotemporal Regulation of Ligand Trafficking and TLR9 Activation by PIEZO1 in Human Plasmacytoid Dendritic Cells
doi: 10.34133/research.1067
Figure Lengend Snippet: CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by ELISA. n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
Article Snippet: Concentration of IFN-α and TNF-α in pDC culture supernatants was determined using
Techniques: Membrane, Confocal Microscopy, Enzyme-linked Immunosorbent Assay, Expressing, Control, Knockdown, Gene Expression