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Mabtech Inc double antibody sandwich elisa kit
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Cytokine profiling <t>of</t> <t>IFN-γ</t> and IL-5 secreted by splenocytes re-stimulated with recombinant proteins: A–B) Re-stimulation with Nef; C–D) Re-stimulation with NT-Hsp70-Nef, CT-Hsp70-Nef, or IL-15. Cytokine levels were measured by sandwich <t>ELISA</t> in duplicate assays per group (ns: non-significant; * p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Sandwich Elisa Kits, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
Sandwich Enzyme Linked Immunosorbent Assay Elisa, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
Mabtech Iris Reader, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
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CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by <t>ELISA.</t> n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.
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Image Search Results


Cytokine profiling of IFN-γ and IL-5 secreted by splenocytes re-stimulated with recombinant proteins: A–B) Re-stimulation with Nef; C–D) Re-stimulation with NT-Hsp70-Nef, CT-Hsp70-Nef, or IL-15. Cytokine levels were measured by sandwich ELISA in duplicate assays per group (ns: non-significant; * p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Journal: PLOS One

Article Title: Simultaneous efficacy of IL-15 and the HSP70 fragments in increasing immunostimulatory effects of a HIV-1 DNA vaccine candidate against SCR HIV-1

doi: 10.1371/journal.pone.0336042

Figure Lengend Snippet: Cytokine profiling of IFN-γ and IL-5 secreted by splenocytes re-stimulated with recombinant proteins: A–B) Re-stimulation with Nef; C–D) Re-stimulation with NT-Hsp70-Nef, CT-Hsp70-Nef, or IL-15. Cytokine levels were measured by sandwich ELISA in duplicate assays per group (ns: non-significant; * p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The concentrations of interferon-gamma (IFN-γ) and interleukin-5 (IL-5) in culture supernatants were quantified using commercial sandwich ELISA kits [Mabtech; mouse IL-5 (HRP): 3391-1H-6/3391-1H-20; mouse IFN-gamma: 3321-1H-6] following the manufacturer’s guidelines.

Techniques: Recombinant, Sandwich ELISA

Data represent sandwich ELISA results performed in triplicate per sample (ns: non-significant; * p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Journal: PLOS One

Article Title: Simultaneous efficacy of IL-15 and the HSP70 fragments in increasing immunostimulatory effects of a HIV-1 DNA vaccine candidate against SCR HIV-1

doi: 10.1371/journal.pone.0336042

Figure Lengend Snippet: Data represent sandwich ELISA results performed in triplicate per sample (ns: non-significant; * p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: The concentrations of interferon-gamma (IFN-γ) and interleukin-5 (IL-5) in culture supernatants were quantified using commercial sandwich ELISA kits [Mabtech; mouse IL-5 (HRP): 3391-1H-6/3391-1H-20; mouse IFN-gamma: 3321-1H-6] following the manufacturer’s guidelines.

Techniques: Sandwich ELISA

CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by ELISA. n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.

Journal: Research

Article Title: Spatiotemporal Regulation of Ligand Trafficking and TLR9 Activation by PIEZO1 in Human Plasmacytoid Dendritic Cells

doi: 10.34133/research.1067

Figure Lengend Snippet: CpGA-induced membrane tension activates PIEZO1 to selectively enhance IFN-α production by pDCs. (A) Representative confocal microscopy images showing intracellular PIEZO1 distribution in untreated pDCs and those treated with CpGA or CpGB. (B and C) IFN-α production in pDC culture supernatants following stimulation with CpGA or CpGB (B), and CpGA with increasing doses of GsMTx4 (C), measured by ELISA. n = 3 to 4. (D and E) IFN-α secretion (D) and IFNA1 mRNA expression (E) in control and PIEZO1-knockdown (PIEZO1 KD) pDCs treated with or without CpGA. n = 4 to 7. (F) IFN-α production by pDCs stimulated with CpGB in the presence or absence of Yoda1. n = 7. (G and H) IFN-α secretion (G) and IFNA1 gene expression (H) in control and PIEZO1 KD pDCs stimulated with CpGB with or without Yoda1 were measured. n = 6 to 7. Data represent median. Statistical analysis was performed using Student’s t test.

Article Snippet: Concentration of IFN-α and TNF-α in pDC culture supernatants was determined using sandwich enzyme-linked immunosorbent assay (ELISA) (Mabtech, Sweden) according to the manufacturer’s protocol.

Techniques: Membrane, Confocal Microscopy, Enzyme-linked Immunosorbent Assay, Expressing, Control, Knockdown, Gene Expression